ATF: Biomed ENG

Takahashi Lab

 
 

Radial gradients of myoglobin light absorption in an isolated single cardiomyocyte with elevated mitochondrial respiration, indicating steep gradients of oxygen supply from the sarcolemma to the cell core.

  1. •Takahashi E, Doi K: Visualization of oxygen level inside a single cardiac myocyte. Am J Physiol 268: H2561-H2568, 1995.

  2. •Takahashi E, Doi K: Regulation of oxygen diffusion to hypoxic cardiac myocytes. Am J Physiol 271: H1734-H1739, 1996.

  3. •Takahashi E, Sato K, Endoh H, Xu ZL, Doi K: Direct observation of radial intracellular Po2 gradients in a single cardiomyocyte of the rat. Am J Physiol 275: H225-H233, 1998.

  4. •Takahashi E, Endoh H, Doi K: Intracellular gradients of O2 supply to mitochondria in actively respiring single cardiomyocyte of rats. Am J Physiol 276: H718-H724, 1999.

  5. •Takahashi E, Endoh H, Doi K: Visualization of myoglobin facilitated mitochondrial O2 delivery in a single isolated cardiomyocyte. Biophys J 78: 3252-3259, 2000.

Anoxic cell core imaged as regional increases in mitochondrial NADH autofluorescence.

Bioimaging of oxygen metabolism in cells


We have developed a novel bioimaging technique that allows imaging of oxygen concentration, mitochondrial oxygen metabolism, and ATP concentration within an isolated single cardiomyocyte with a subcellular spatial resolution. Using this technique, we demonstrated heterogeneities of oxygen metabolism and anoxic cell core when the mitochondrial respiration was stimulated at physiological extracellular oxygen levels.

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